Fruit Fly Culture Guide

Materials

• Fruit fly culture mix
• Fruit fly culture cups with mesh lids
• Excelsior
• Fruit fly culture that is currently producing (Drosophila melanogaster or Drosophila hydei)

Directions

1. Buy fly culture mix (I.e., Repashy Superfly, FrogDaddy Premium Fruit Fly Media), fly culture cups with mesh lids, Excelsior, and a producing fruit fly culture.
2. Add media mix to an empty culture cup, per media instructions.
3. Mix with boiling water per media instructions. Let cool 1-2 hours.
4. Add a handful of Excelsior.
5. Add 50-100 fruit flies from the producing mix.
6. Wait 2 weeks if melanogaster, 3-4 weeks if hydei before you begin using culture for feeding.